AJIC: American Journal of Infection Control
Volume 38, Issue 3 , Pages 195-198 , April 2010

Detection of Aspergillus fumigatus by quantitative polymerase chain reaction in air samples impacted on low-melt agar

  • Anne-Pauline Bellanger, PharmD

      Affiliations

    • Department of Parasitology-Mycology, University Hospital Jean Minjoz, Besançon, France
    • Research Team Chrono-Environnement, Université de Franche Comté, Besançon, France
    • Corresponding Author InformationAddress correspondence to Anne-Pauline Bellanger, PharmD, Department of Parasitology-Mycology, University Hospital, Besançon, France.
    • ,
  • Gabriel Reboux, PhD

      Affiliations

    • Department of Parasitology-Mycology, University Hospital Jean Minjoz, Besançon, France
    • Research Team Chrono-Environnement, Université de Franche Comté, Besançon, France
    • ,
  • Jean-Benjamin Murat, PharmD

      Affiliations

    • Department of Parasitology-Mycology, University Hospital Jean Minjoz, Besançon, France
    • Research Team Chrono-Environnement, Université de Franche Comté, Besançon, France
    • ,
  • Valerie Bex, PhD

      Affiliations

    • Hygiene Laboratory of Paris, Paris, France
    • ,
  • Laurence Millon, PharmD, PhD

      Affiliations

    • Department of Parasitology-Mycology, University Hospital Jean Minjoz, Besançon, France
    • Research Team Chrono-Environnement, Université de Franche Comté, Besançon, France

References 

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  2. Walsh TJ, Anaissie EJ, Denning DW, Herbrecht R, Kontoyiannis DP, Marr KA, et al. Treatment of aspergillosis: clinical practice guidelines of the Infectious Diseases of America. Clin Infect Dis. 2008;46:327–360
  3. Perfect JR, Cox GM, Lee JY, Kauffman CA, de Repentigny L, Chapman SW. The impact of culture isolation of Aspergillus species: a hospital-based survey of aspergillosis. Clin Infect Dis. 2001;33:1824–1833
  4. Falvey DG, Streifel AJ. Ten-year air sample analysis of Aspergillus prevalence in a university hospital. J Hosp Infect. 2007;67:35–41
  5. Rupp ME, Iwen PC, Tyner LK, Marion N, Reed E, Anderson JR. Routine sampling of air for fungi does not predict risk of invasive aspergillosis in immunocompromised patients. J Hosp Infect. 2008;68:270–271
  6. Morrison J, Yang C, Lin KT, Haugland RA, Neely AN, Vesper AJ. Monitoring Aspergillus species by quantitative PCR during construction of a multi-story hospital building. J Hosp Infect. 2004;57:85–87
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  8. Haugland RA, Varma M, Wymer LJ, Vesper SJ. Quantitative PCR analysis of selected Aspergillus, Penicillium, and Paecilomyces species. Syst Appl Microbiol. 2004;27:198–210
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  10. Keswani J, Kashon ML, Chen BT. Evaluation of interference to conventional and real-time PCR for detection and quantification of fungi in dust. J Environ Monit. 2005;7:311–318
  11. Vesper SJ, Haugland RA, Rogers ME, Neely AN. Opportunistic Aspergillus pathogens measured in home and hospital tap water by quantitative PCR (QPCR). J Water Health. 2007;5:427–431
  12. Neely AN, Gallardo V, Barth E, Haugland RA, Warden GD, Vesper SJ. Rapid monitoring by quantitative polymerase chain reaction for pathogenic Aspergillus during carpet removal from a hospital. Infect Control Hosp Epidemiol. 2004;25:350–352
  13. Cruz P, Buttner MP. Analysis of bioaerosol samples. In:  Hurst CJ,  Crawford RL,  Garland JL,  Lipson DA,  Mills AL,  Stetzenbach LD editor. Manual of environmental microbiology. 3rd ed. Washington, DC: ASM Press; 2007;p. 952–960
  14. Rivera A, Van Epps HL, Hohl TM, Rizzuto G, Pamer EG. Distinct CD4+ T-cell response to live and heat-inactivated Aspergillus fumigatus conidia. Infect Immun. 2005;73:7170–7179
  15. Wasylnka JA, Hissen AH, Wan AN, Moore MM. Intracellular and extracellular growth of Aspergillus fumigatus. Med Mycol. 2005;43(Suppl 1):S27–S30
  16. Vesper S, McKinstry C, Hartmann C, Neace M, Yoder S, Vesper A. Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA). J Microbiol Methods. 2008;72:180–184
  17. Spicer R, Gangloff H. Establishing site-specific reference levels for fungi in outdoor air for building evaluation. J Occup Environ Hyg. 2005;2:257–266
  18. Spicer RC, Gangloff HJ. Bioaerosol data distribution: probability and implications for sampling in evaluating problematic buildings. Appl Occup Environ Hyg. 2003;18:584–590
  19. Vesper SJ, Wong W, Kuo CM, Pierson DL. Mold species in dust from the International Space Station identified and quantified by mold-specific quantitative PCR. Res Microbiol. 2008;159:432–435

 Conflicts of interest: None to report

PII: S0196-6553(09)00822-0

doi: 10.1016/j.ajic.2009.08.003

AJIC: American Journal of Infection Control
Volume 38, Issue 3 , Pages 195-198 , April 2010

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