Major article| Volume 36, ISSUE 8, P559-563, October 2008

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Ozone gas is an effective and practical antibacterial agent


      Bacterial infections continue to pose a threat to health in many institutional and communal settings, and epidemics are frequent. Current control measures are clearly inadequate; thus, there is a need for a simple, effective, and safe way to decontaminate surfaces.


      We evaluated the efficacy of a portable ozone-generating machine, equipped with a catalytic converter and an accessory humidifier, to inactivate 15 different species of medically important bacteria.


      An ozone dosage of 25 ppm for 20 minutes, with a short burst of humidity in excess of 90% relative humidity, was able to inactivate more than 3 log10 colony-forming units of most of the bacteria, including Acinetobacter baumannii, Clostridium difficile, and methicillin-resistant Staphylococcus aureus, in both in a laboratory test system and simulated field conditions. In many cases, complete eradication was achieved. Dried and wet samples were equally vulnerable to the ozone. Inactivation of bacterial samples dried onto soft surfaces (eg, fabric, cotton, filter paper) were comparable with that observed for samples on plastic.


      The ozone generator can provide a valuable decontamination tool for the removal of bacteria in many institutional and communal settings, including hospitals and other health care institutions.
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        • Aslam S.
        • Hamill R.J.
        • Musher D.M.
        Treatment of Clostridium difficile–associated disease: old therapies and new strategies.
        Lancet Infect Dis. 2005; 5: 549-557
        • Bartlett J.G.
        The new epidemic of Clostridium difficile–associated enteric disease.
        Ann Intern Med. 2006; 145: 758-764
        • Cloud J.
        • Kelly C.P.
        Update on Clostridium difficile–associated disease.
        Curr Opin Gastroenterol. 2007; 23: 4-9
        • de Boer H.E.L.
        • van Elzelingen-Dekker C.M.
        • van Reeberg-Verberg C.M.F.
        • Spanjaard L.
        Use of gaseous ozone for eradication of methicillin-resistant Staphylococcus aureus from the home environment of a colonized hospital employee.
        Infect Control Hosp Epidemiol. 2006; 27: 1120-1122
        • Robenshtok E.
        • Paul M.
        • Leibovici L.
        • Fraser A.
        • Pitlik S.
        • Ostfeld I.
        • et al.
        The significance of Acinetobacter baumannii bacteraemia compared with Klebsiella pneumoniae bacteraemia: risk factors and outcomes.
        J Hosp Infect. 2006; 64: 282-287
        • Falagas M.E.
        • Bliziotis I.A.
        • Siempos I.I.
        Attributable mortality of Acinobacter baumannii infections in critically ill patients: a systemic review of matched cohort and case-control studies.
        Crit Care. 2006; 10: 1-8
        • Scott P.
        • Deye G.
        • Srinivasan A.
        • Murray C.
        • Moran K.
        • Hultan E.
        • et al.
        An outbreak of multidrug-resistant Acinetobacter baumanii–calcoaceticus complex infection in the US military health care system associated with military operations in Iraq.
        Clin Infect Dis. 2007; 44: 1577-1584
        • Kerr K.G.
        • Beggs C.B.
        • Dean S.G.
        • Thornton J.
        • Donnelly J.K.
        • Todd N.J.
        • et al.
        Air ionization and colonization/infection with methicillin-resistant Staphylococcus aureus and Acinetobacter species in an intensive care unit.
        Intensive Care Med. 2006; 32: 315-317
        • Lopman B.A.
        • Reacher M.H.
        • Vipond I.B.
        • Hill D.
        • Perry C.
        • Halladay T.
        • et al.
        Epidemiology and cost of nosocomial gastroenteritis, Avon, England, 2002–2003.
        Emerg Infect Dis. 2004; 10: 1827-1834
        • Wullt M.
        • Odenholt I.
        • Walder M.
        Activity of three disinfectants and acidified nitrite against Clostridium difficile spores.
        Infect Control Hosp Epidemiol. 2003; 24: 765-768
        • Omidbakhsh N.
        • Sattar S.A.
        Broad-spectrum microbicidal activitiy, toxicologic assessment, and materials compatibility of a new generation of accelerated hydrogen peroxide–based environmental surface disinfectant.
        Am J Infect Control. 2006; 34: 251-257
        • French G.L.
        • Otter J.A.
        • Shannon K.P.
        • Adams N.M.T.
        • Watling D.
        • Parks M.J.
        Tackling contamination of the hospital environment by methicillin-resistant Staphylococcus aureus (MRSA): a comparison between conventional terminal cleaning and hydrogen peroxide vapor decontamination.
        J Hosp Infect. 2004; 57: 31-37
        • Fan L.
        • Song J.
        • Hildebrand P.D.
        • Forney C.F.
        Interaction of ozone and negative air ions to control microorganisms.
        J Appl Microbiol. 2002; 93: 144-148
        • Kowalski W.J.
        • Bahnfleth W.P.
        • Striebig B.A.
        • Whittam T.S.
        Demonstration of a hermetic airborne ozone disinfection system: studies on E coli.
        AIHA J. 2003; 64: 222-227
        • Gaunt L.
        • Higgins S.
        • Hughes J.
        Decontamination of surface-borne bacteria by ionized antimicrobial vapours.
        J Electrostatics. 2005; 63: 809-814
        • Fletcher L.A.
        • Gaunt L.F.
        • Beggs C.B.
        • Shepherd S.A.
        • Sleigh P.A.
        • Noakes C.J.
        • et al.
        Bactericidal action of positive and negative ions in air.
        BMC Microbiol. 2007; 7: 1-9
        • Hudson J.B.
        • Sharma M.
        • Petric M.
        Inactivation of norovirus by ozone gas in conditions relevant to health care.
        J Hosp Infect. 2007; 66: 40-45
        • Jarvis W.R.
        Selected aspects of the socioeconomic impact of nosocomial infections.
        Infect Control Hosp Epidemiol. 1996; 17: 552-557
        • Berrington A.W.
        • Pedler S.J.
        Investigation of gaseous ozone for MRSA decontamination of hospital side rooms.
        J Hosp Infect. 1998; 40: 61-65
        • Li C.-S.
        • Wang Y.-C.
        Surface germicidal effects of ozone for microorganisms.
        AIHA J. 2003; 64: 533-537