Background
Klebsiella pneumoniae carbapenemase-producing K pneumoniae (KPC-KP) outbreaks have been reported in many countries, including Brazil. The incidence
of KPC-KP infection has increased in the first semester of 2011 in Curitiba, the capital
of Parana, in Southern Brazil.The aim of this study was to characterize the infections
and clonal diversity of KPC-KP isolates from several institutions in Curitiba.
Methods
KPC-KP from several clinical samples and rectal swabs taken between April 2010 and
July 2012 were included. One isolate per patient was evaluated. All isolates were
submitted to polymerase chain reaction (PCR) for blaKPC. The genetic relatedness was evaluated using strain clustering by an automated repetitive
extragenic palindromic (rep) PCR-based typing system.
Results
There were 641 samples that were positive for K pneumoniae carbapenemase-2 carbapenemase. There were 129 samples randomly selected for clonality
evaluation. PCR and strain clustering by the automated rep PCR-based typing system
identified 7 clones (A-C and E-H). Clone E was identified in only 1 hospital, and
all other clones were found in >2 hospitals. Clones C and G were the most disseminated
among hospitals. The infection and colonization occurred in 14 out of the 32 main
hospitals in town. Similar clones were found in 2 hospitals that are administered
by the same group. Another clone (H) was found in 2 hospitals receiving patients from
the same municipal emergency unit.
Conclusion
The KPC-KP outbreak in Curitiba is polyclonal, and the source is unknown. Some hospitals
share the same clones.
Key Words
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Article info
Publication history
Published online: December 23, 2014
Footnotes
Funding/support: None.
Conflicts of interest: None to report.
Identification
Copyright
© 2015 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.
