Highlights
- •Environmental investigation in the context of an outbreak can be an arduous task.
- •If it is Klebsiella oxytoca sinks/washbasins and drains must be sampled.
- •It is necessary perform sampling always before cleaning the area.
- •It is important to concentrate the water samples to maximize bacterial recovery.
- •For cultures, it is helpful to use an enrichment broth which favors growth.
We describe a method for investigating wet foci in the hospital environment of a nosocomial
outbreak due to carbapenem-resistant Klebsiella oxytoca. By searching in areas that are usually inaccessible, by immersing sampling swabs
in thioglycolate broth, and by concentrating the sampled water, reservoirs of infection
that would have gone unnoticed can be detected.
Key Words
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References
- Guidelines for environmental infection control in health-care facilities.2003: 88-140
- Outbreak of extended-spectrum ß-lactamase–producing Klebsiella oxytoca infections associated with contaminated handwashing sinks.Emerg Infect Dis. 2012; 18: 1242-1247
- Wastewater drainage system as an occult reservoir in a protracted clonal outbreak due to metallo-ß-lactamase producing Klebsiella oxytoca.Clin Microbiol Infect. 2013; 19: E490-8
- Isolation of multidrug-resistant Klebsiella oxytoca carrying blaIMP-8, associated with OXY hyperproduction, in the intensive care unit of a community hospital in Spain.J Antimicrob Chemother. 2010; 65: 1071-1073
- Molecular epidemiology of ceftazidime-resistant Gram-negative bacilli on inanimate surfaces and their role in cross-transmission during non-outbreak periods.J Clin Microbiol. 1999; 37: 3065-3067
- Drainage systems, an occluded source of sanitation-related outbreaks.Arch Public Health. 2015; 73: 8
- Environmental contamination with extended-spectrum ß-lactamase: is there any difference between Escherichia coli and Klebsiella spp?.Am J Infect Control. 2012; 40: 845-848
- Environmental contamination by carbapenem-resistant Enterobacteriaceae.J Clin Microbiol. 2012; 51: 177-181
- Comparison of two sampling methods for the detection of Gram-positive and Gram-negative bacteria in the environment: moistened swabs versus Rodac plates.Int J Hyg Environ Health. 2001; 203: 245-248
- Search and you will find: detecting extended-spectrum ß-lactamase-producing Klebsiella pneumoniae from a patient's immediate environment.Infect Control Hosp Epidemiol. 2013; 34: 534-536
Article info
Publication history
Published online: June 13, 2016
Footnotes
Conflicts of Interest: None to report.
Identification
Copyright
© 2016 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.
