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Environmental sampling protocol devised and implemented to resolve a nosocomial outbreak due to carbapenem-resistant Klebsiella oxytoca

      Highlights

      • Environmental investigation in the context of an outbreak can be an arduous task.
      • If it is Klebsiella oxytoca sinks/washbasins and drains must be sampled.
      • It is necessary perform sampling always before cleaning the area.
      • It is important to concentrate the water samples to maximize bacterial recovery.
      • For cultures, it is helpful to use an enrichment broth which favors growth.
      We describe a method for investigating wet foci in the hospital environment of a nosocomial outbreak due to carbapenem-resistant Klebsiella oxytoca. By searching in areas that are usually inaccessible, by immersing sampling swabs in thioglycolate broth, and by concentrating the sampled water, reservoirs of infection that would have gone unnoticed can be detected.

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