Brief Report| Volume 48, ISSUE 7, P843-845, July 2020

Longitudinal study on the detection of Clostridioides difficile at the Fargo Veteran Affairs Community Living Center

Published:November 19, 2019DOI:


      • Clostridiodes Difficile environmental detection in long-term care facility.
      • Detection of Clostridiodes Difficile Spores using the Clostridiodes Banana Broth.
      • Environmental Contamination and Persistence of Clostidiodes Difficile Spores could unknowingly be passed to patients and staff.
      Our study used C Diff Banana Broth to evaluate the occurrence of positive Clostridioides difficile spores in new and preexisting hospital rooms. C difficile incidence was 5.5%. Analysis using multiple linear regression found that rooms with contact precautions in place were significant predictors of a positive sample (P ≤ .001). Room occupancy was not a significant predictor (P = .544). Thus it could be interpreted that the environment can be a significant carrier for C difficile.

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        • Centers for Disease Control and Prevention
        Antibiotic resistance threats in the United States.
        2013 (Available from:) (Accessed September 5, 2019)
        • Tarrant J
        • Jenkins RO
        • Laird KT
        From ward to washer: the survival of Clostridium difficile spores on hospital bed sheets through a commercial UK NHS healthcare laundry process.
        Infect Control Hosp Epidemiol. 2018; 39: 1406-1411
        • Shams AM
        • Rose LJ
        • Edwards JR
        • Cali S
        • Harris AD
        • Jacob JT
        • et al.
        Assessment of the overall and multidrug-resistant organism bioburden on environmental surfaces in healthcare facilities.
        Infect Control Hosp Epidemiol. 2016; 37: 1426-1432
        • Barker AK
        • Ngam C
        • Musuuza JS
        • Vaughn VM
        • Safdar N
        Reducing Clostridium difficile in the inpatient setting: a systematic review of the adherence to and effectiveness of C.
        difficile prevention bundles. Infect Control Hosp Epidemiol. 2017; 38: 639-650
        • Cadnum JL
        • Hurless KN
        • Deshpande A
        • Nerandzic MM
        • Kundrapu S
        • Donskey CJ
        Sensitive and selective culture medium for detection of environmental Clostridium difficile isolates without requirement for anaerobic culture conditions.
        J Clin Microbiol. 2014; 52: 3259-3263
      1. Environmental Management Service. Sanitation Procedure Guide 2016. Dallas (TX): Department of Veteran Affairs; 2016:62-77.Available from: Accessed November 7, 2019.

        • Lax S
        • Smith D
        • Sangwan N
        • Handley KM
        • Larsen P
        • Richardson M
        • et al.
        Colonization and succession of hospital-associated microbiota.
        Sci Transl Med. 2017; 9
        • Choe YJ
        • Smit MA
        • Mermel LA.
        Seasonality of respiratory viruses and bacterial pathogens.
        Antimicrob Resist Infect Control. 2019; 8: 125