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A review of wipes used to disinfect hard surfaces in health care facilities

      Highlights

      • Compatibility of disinfectants with material from which wipes are made is important.
      • Contact times on product labels should be evidence-based and practical.
      • Periodically test the concentration of disinfectants requiring dilution or mixing.
      • More detailed instructions for use are needed for ready-to-use (RTU) wipes.
      • An internationally accepted method for testing wipe efficacy is needed.

      Abstract

      Background

      Despite a plethora of wipes available for use in health care facilities, there is a paucity of articles describing wipe composition, potential interactions between wipes and disinfectants, the manner in which wipes are used, and their relative efficacy. The purpose of this article is to provide an in-depth review of wipes used for disinfection of hard surfaces in health care settings.

      Methods

      Comprehensive searches of the Pubmed database and Internet were conducted, and articles published from 1953 through September 2019 and pertinent on-line documents were reviewed. Bibliographies of relevant articles were reviewed.

      Results

      Wipes vary considerably in their composition, and the disinfectants with which they are used. With reusable dry wipes, the ratio of wipe material to disinfectant and the amount of disinfectant absorbed by the wipe and delivered to surfaces is difficult to standardize, which may affect their efficacy. The manner in which wipes are used by health care personnel is highly variable, due in part to insufficient instructions for use and inadequate education of relevant personnel.

      Conclusions

      Additional research is needed regarding the best practices for using different types of wipes, improved methods for educating staff, and establishing the relative efficacy of wipes in reducing environmental contamination and health care-associated infections.

      Key Words

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      References

        • Weber DJ
        • Anderson D
        • Rutala WA
        The role of the surface environment in healthcare-associated infections.
        Curr Opin Infect Dis. 2013; 26: 338-344
        • Otter JA
        • Yezli S
        • Salkeld JA
        • French GL
        Evidence that contaminated surfaces contribute to the transmission of hospital pathogens and an overview of strategies to address contaminated surfaces in hospital settings.
        Am J Infect Control. 2013; 41: S6-11
        • Hayden MK
        • Blom DW
        • Lyle EA
        • Moore CG
        • Weinstein RA
        Risk of hand or glove contamination after contact with patients colonized with vancomycin-resistant enterococcus or the colonized patient's environment.
        Infect Control Hosp Epidemiol. 2008; 29: 149-154
        • Stiefel U
        • Cadnum JL
        • Eckstein BC
        • Guerrero DM
        • Tima MA
        • Donskey CJ
        Contamination of hands with methicillin-resistant Staphylococcus aureus after contact with environmental surfaces and after contact with the skin of colonized patients.
        Infect Control Hosp Epidemiol. 2011; 32: 185-187
        • Mitchell BG
        • Dancer SJ
        • Anderson M
        • Dehn E
        Risk of organism acquisition from prior room occupants: a systematic review and meta-analysis.
        J Hosp Infect. 2015; 91: 211-217
        • Hayden MK
        • Bonten MJ
        • Blom DW
        • Lyle EA
        • van de Vijver DA
        • Weinstein RA
        Reduction in acquisition of vancomycin-resistant Enterococcus after enforcement of routine environmental cleaning measures.
        Clin Infect Dis. 2006; 42: 1552-1560
        • Donskey CJ
        Does improving surface cleaning and disinfection reduce health care-associated infections?.
        Am J Infect Control. 2013; 41: S12-S19
        • Rutala WA
        • Weber DJ.
        Monitoring and improving the effectiveness of surface cleaning and disinfection.
        Am J Infect Control. 2016; 44: e69-e76
        • Sattar SA
        • Maillard JY.
        The crucial role of wiping in decontamination of high-touch environmental surfaces: review of current status and directions for the future.
        Am J Infect Control. 2013; 41: S97-104
        • Rutala WA
        • Weber DJ.
        Selection of the ideal disinfectant.
        Infect Control Hosp Epidemiol. 2014; 35: 855-865
      1. RutalaWA, WeberDJ, the Healthcare Infection Control Practices Advisory Committee. Guideline for disinfection and sterilization in healthcare facilities, 2008. Available at: https://www.cdc.gov/infectioncontrol/guidelines/disinfection/. Accessed June 13, 2020.

        • Public Health Ontario
        Best practices for environmental cleaning for prevention and control of infections in all health care settings..
        3rd ed. 2018 (Available at:) (Accessed June 13, 2020)
        • Moore G
        • Griffith C.
        A laboratory evaluation of the decontamination properties of microfibre cloths.
        J Hosp Infect. 2006; 64: 379-385
        • Smith DL
        • Gillanders S
        • Holah JT
        • Gush C
        Assessing the efficacy of different microfibre cloths at removing surface micro-organisms associated with healthcare-associated infections.
        J Hosp Infect. 2011; 78: 182-186
        • MacDougall KD
        • Morris C.
        Optimizing disinfectant application in healthcare facilities.
        Infect Control Today. 2006; 10: 62-67
        • Boyce JM
        • Sullivan L
        • Booker A
        • Baker J
        Quaternary ammonium disinfectant issues encountered in an environmental services department.
        Infect Control Hosp Epidemiol. 2016; 37: 340-342
        • Sifuentes LY
        • Gerba CP
        • Weart I
        • Engelbrecht K
        • Koenig DW
        Microbial contamination of hospital reusable cleaning towels.
        Am J Infect Control. 2013; 41: 912-915
        • Bergen LK
        • Meyer M
        • Hog M
        • Rubenhagen B
        • Andersen LP
        Spread of bacteria on surfaces when cleaning with microfibre cloths.
        J Hosp Infect. 2009; 71: 132-137
      2. Walleser M.Cleanroom wipe use, protocol, and ISO class standards. Available at: https://blog.gotopac.com/2017/11/20/the-guide-to-low-lint-wipes-for-cleanroom-wipedown-the-differences-between-woven-and-nonwoven-cleanroom-wipes/. Accessed June 13, 2020.

        • Pu Y
        • Zheng J
        • Chen F
        • et al.
        Preparation of olypropylenemicro and nanofibers by electrostatic-assisted melt blown and their application.
        Polymers (Basel). 2018; 10 (Available at:) (Accessed June 13, 2020): 959
        • Chellamani KP
        • VigneshBalaji RS
        • Veerasubramanian D
        Medical textiles: the spunlace process and its application possibilities for hygiene textiles.
        J Acad Indus Res. 2013; 1: 735-739
        • Edwards NWM
        • Best EL
        • Connell SD
        • et al.
        Role of surface energy and nano-roughness in the removal efficiency of bacterial contamination by nonwoven wipes from frequently touched surfaces.
        Sci Technol Adv Mater. 2017; 18: 197-209
        • Wren MW
        • Rollins MS
        • Jeanes A
        • Hall TJ
        • Coen PG
        • Gant VA
        Removing bacteria from hospital surfaces: a laboratory comparison of ultramicrofibre and standard cloths.
        J Hosp Infect. 2008; 70: 265-271
        • Rutala WA
        • Gergen MF
        • Weber DJ
        Efficacy of different cleaning and disinfection methods against Clostridium difficile spores: importance of physical removal versus sporicidal inactivation.
        Infect Control Hosp Epidemiol. 2012; 33: 1255-1258
        • Erasmus V
        • Daha TJ
        • Brug H
        • et al.
        Systematic review of studies on compliance with hand hygiene guidelines in hospital care.
        Infect Control Hosp Epidemiol. 2010; 31: 283-294
        • West AM
        • Nkemngong CA
        • Voorn MG
        • et al.
        Surface area wiped, product type, and target strain impact bactericidal efficacy of ready-to-use disinfectant towelettes.
        Antimicrob Resist Infect Control. 2018; 7: 122
        • Diab-Elschahawi M
        • Assadian O
        • Blacky A
        • et al.
        Evaluation of the decontamination efficacy of new and reprocessed microfiber cleaning cloth compared with other commonly used cleaning cloths in the hospital.
        Am J Infect Control. 2010; 38: 289-292
        • Bhalla A
        • Pultz NJ
        • Gries DM
        • et al.
        Acquisition of nosocomial pathogens on hands after contact with environmental surfaces near hospitalized patients.
        Infect Control Hosp Epidemiol. 2004; 25: 164-167
        • Goodman ER
        • Platt R
        • Bass R
        • Onderdon AB
        • Yokoe DS
        • Huang SS
        Impact of an environmental cleaning intervention on the presence of methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci on surfaces in intenstive care unit rooms.
        Infect Control Hosp Epidemiol. 2008; 29: 593-599
        • Hota B
        • Blom DW
        • Lyle EA
        • Weinstein RA
        • Hayden MK
        Interventional evaluation of environmental contamination by vancomycin-resistant enterococci: failure of personnel, product, or procedure?.
        J Hosp Infect. 2009; 71: 123-131
        • Blazejewski C
        • Wallet F
        • Rouze A
        • et al.
        Efficiency of hydrogen peroxide in improving disinfection of ICU rooms.
        Crit Care. 2015; 19: 30
        • Lorenzi N.
        Developments in disinfection.
        Healthc Facil Manag. 2015; 28: 37-40
        • Williams GJ
        • Denyer SP
        • Hosein IK
        • Hill DW
        • Maillard JY
        Use of sodium dichloroisocyanurate for floor disinfection.
        J Hosp Infect. 2009; 72: 279-281
        • Ramm L
        • Siani H
        • Wesgate R
        • Maillard JY
        Pathogen transfer and high variability in pathogen removal by detergent wipes.
        Am J Infect Control. 2015; 43: 724-728
        • Miller R
        • Simmons S
        • Dale C
        • Stachowiak J
        • Stibich M
        Utilization and impact of a pulsed-xenon ultraviolet room disinfection system and multidisciplinary care team on Clostridium difficile in a long-term acute care facility.
        Am J Infect Control. 2015; 43: 1350-1353
        • Peterson LR
        • Boehm S
        • Beaumont JL
        • et al.
        Reduction of methicillin-resistant Staphylococcus aureus infection in long-term care is possible while maintaining patient socialization: a prospective randomized clinical trial.
        Am J Infect Control. 2016; 44: 1622-1627
        • Gerba CP
        • Lopez GU
        • Ikner LA
        Distribution of bacteria in dental offices and the impact of hydrogen peroxide disinfecting wipes.
        J Dent Hyg. 2016; 90: 354-361
        • Sebastiani FR
        • Dym H
        • Kirpalani T
        Infection control in the dental office.
        Dent Clin North Am. 2017; 61: 435-457
        • Nandy P
        • Lucas AD
        • Gonzalez EA
        • Hitchins VM
        Efficacy of commercially available wipes for disinfection of pulse oximeter sensors.
        Am J Infect Control. 2016; 44: 304-310
        • Sarmaga D
        • Dubois JA
        • Lyon ME
        Evaluation of different disinfectants on the performance of an on-meter dosed amperometric glucose-oxidase-based glucose meter.
        J Diabetes SciTechnol. 2011; 5: 1449-1452
        • Jencson AL
        • Cadnum JL
        • Wilson BM
        • Donskey CJ
        Spores on wheels: wheelchairs are a potential vector for dissemination of pathogens in healthcare facilities.
        Am J Infect Control. 2019; 47: 459-461
        • Lieberman MT
        • Madden CM
        • Ma EJ
        • Fox JG
        Evaluation of 6 methods for aerobic bacterial sanitization of smartphones.
        J Am Assoc Lab Anim Sci. 2018; 57: 24-29
        • Howell V
        • Thoppil A
        • Mariyaselvam M
        • et al.
        Disinfecting the iPad: evaluating effective methods.
        J Hosp Infect. 2014; 87: 77-83
        • Rutala WA
        • White MS
        • Gergen MF
        • Weber DJ
        Bacterial contamination of keyboards: efficacy and functional impact of disinfectants.
        Infect Control Hosp Epidemiol. 2006; 27: 372-377
        • Codish S
        • Toledano R
        • Novack V
        • Sherf M
        • Borer A
        Effectiveness of stringent decontamination of computer input devices in the era of electronic medical records and bedside computing: a randomized controlled trial.
        Am J Infect Control. 2015; 43: 644-646
        • Quon JS
        • Dilauro M
        • Ryan JG
        Disinfection of the radiologist workstation and radiologist hand hygiene: a single institution practice quality improvement project.
        Can Assoc Radiol J. 2017; 68: 270-275
        • Weber DJ
        • Rutala WA
        • Sickbert-Bennett E
        Outbreaks associated with contaminated antiseptics and disinfectants.
        Antimicrob Agents Chemother. 2007; 51: 4217-4224
        • Wiemken TL
        • Curran DR
        • Pacholski EB
        • et al.
        The value of ready-to-use disinfectant wipes: compliance, employee time, and costs.
        Am J Infect Control. 2014; 42: 329-330
        • Siani H
        • Wesgate R
        • Maillard JY
        Impact of antimicrobial wipes compared with hypochlorite solution on environmental surface contamination in a health care setting: a double-crossover study.
        Am J Infect Control. 2018; 46: 1180-1187
        • Delaney MB.
        Kick the bucket: one hospital system's journey to reduce Clostridium difficile.
        J Emerg Nurs. 2017; 43: 519-525
        • Kenters N
        • Huijskens EGW
        • de Wit SCJ
        • et al.
        Effectiveness of various cleaning and disinfectant products on Clostridium difficile spores of PCR ribotypes 010, 014 and 027.
        Antimicrob Resist Infect Control. 2017; 6: 54
        • Kenters N
        • Huijskens EGW
        • de Wit SCJ
        • van RJ
        • Voss A
        Effectiveness of cleaning-disinfection wipes and sprays against multidrug-resistant outbreak strains.
        Am J Infect Control. 2017; 45: e69-e73
        • Rutala WA
        • Gergen MF
        • Weber DJ
        Efficacy of improved hydrogen peroxide against important healthcare-associated pathogens.
        Infect Control Hosp Epidemiol. 2012; 33: 1159-1161
        • Cadnum JL
        • Mana TS
        • Jencson A
        • Thota P
        • Kundrapu S
        • Donskey CJ
        Effectiveness of a hydrogen peroxide spray for decontamination of soft surfaces in hospitals.
        Am J Infect Control. 2015; 43: 1357-1359
        • Alhmidi H
        • Koganti S
        • Cadnum JL
        • Rai H
        • Jencson AL
        • Donskey CJ
        Evaluation of a novel alcohol-based surface disinfectant for disinfection of hard and soft surfaces in healthcare facilities.
        Open Forum Infect Dis. 2017; 4https://doi.org/10.1093/ofid/ofx054
        • Wiemken TL
        • Powell W
        • Carrico RM
        • et al.
        Disinfectant sprays versus wipes: applications in behavioral health.
        Am J Infect Control. 2016; 44: 1698-1699
      3. Health Protection Scotland. Literature review and practice recommendations: existing and emerging technologies used for decontamination of the healthcare environment - wipes. 2017. Available at: https://www.hps.scot.nhs.uk/web-resources-container/literature-review-and-practice-recommendations-existing-and-emerging-technologies-used-for-decontamination-of-the-healthcare-environment-wipes/. Accessed June 13, 2020.

        • Rutala WA
        • Weber DJ.
        Disinfection, sterilization, and antisepsis: an overview.
        Am J Infect Control. 2019; 47: A3-A9
        • Sattar SA.
        Promises and pitfalls of recent advances in chemical means of preventing the spread of nosocomial infections by environmental surfaces.
        Am J Infect Control. 2010; 38: S34-S40
        • Gerba CP
        Quaternary ammonium biocides: efficacy in application.
        Appl Environ Microbiol. 2015; 81: 464-469
        • Leas BF
        • Sullivan N
        • Han JH
        • Pegues DA
        • Kaczmarek JL
        • Umscheid CA
        Environmental cleaning for the prevention of healthcare-associated infections.
        Technical Brief No. 22. AHRQ Publication No. 15-EHC020-EF ed. Agency for Healthcare Research and Quality, Rockville, MD2015
        • Ledwoch K
        • Maillard JY.
        Candida auris dry surface biofilm (DSB) for disinfectant efficacy testing.
        Materials. 2018; 12: 18
        • Siani H
        • Cooper C
        • Maillard JY
        Efficacy of "sporicidal" wipes against Clostridium difficile.
        Am J Infect Control. 2011; 39: 212-218
        • Baxa D
        • Shetron-Rama L
        • Golembieski M
        • et al.
        In vitro evaluation of a novel process for reducing bacterial contamination of environmental surfaces.
        Am J Infect Control. 2011; 39: 483-487
        • Tamimi AH
        • Carlino S
        • Gerba CP
        Long-term efficacy of a self-disinfecting coating in an intensive care unit.
        Am J Infect Control. 2014; 42: 1178-1181
        • Schmidt MG
        • Fairey SE
        • Attaway HH
        In situ evaluation of a persistent disinfectant provides continuous decontamination within the clinical environment.
        Am J Infect Control. 2019; 47: 732-734
        • Rutala WA
        • Gergen MF
        • Sickbert-Bennett EE
        • Anderson DJ
        • Weber DJ
        Antimicrobial activity of a continuously active disinfectant against healthcare pathogens.
        Infect Control Hosp Epidemiol. 2019; 40: 1284-1286
        • Ng Wong YK
        • Alhmidi H
        • Mana TSC
        • Cadnum JL
        • Jencson AL
        • Donskey CJ
        Impact of routine use of a spray formulation of bleach on Clostridium difficile spore contamination in non-C difficile infection rooms.
        Am J Infect Control. 2019; 47: 843-845
        • Rutala WA
        • Weber DJ.
        Use of inorganic hypochlorite (bleach) in health-care facilities.
        Clin Microbiol Rev. 1997; 10: 597-610
        • Sattar SA
        • Bradley C
        • Kibbee R
        • et al.
        Disinfectant wipes are appropriate to control microbial bioburden from surfaces: use of a new ASTM standard test protocol to demonstrate efficacy.
        J Hosp Infect. 2015; 91: 319-325
        • Deshpande A
        • Mana TS
        • Cadnum JL
        • et al.
        Evaluation of a sporicidal peracetic acid/hydrogen peroxide-based daily disinfectant cleaner.
        Infect Control Hosp Epidemiol. 2014; 35: 1414-1416
        • Cadnum JL
        • Jencson AL
        • O'Donnell MC
        • Flannery ER
        • Nerandzic MM
        • Donskey CJ
        An increase in healthcare-associated Clostridium difficile infection associated with use of a defective peracetic acid-based surface disinfectant.
        Infect Control Hosp Epidemiol. 2017; 38: 300-305
        • Carling PC
        • Perkins J
        • Ferguson J
        • Thomasser A
        Evaluating a new paradigm for comparing surface disinfection in clinical practice.
        Infect Control Hosp Epidemiol. 2014; 35: 1349-1355
        • Casey ML
        • Hawley B
        • Edwards N
        • Cox-Ganser JM
        • Cummings KJ
        Health problems and disinfectant product exposure among staff at a large multispecialty hospital.
        Am J Infect Control. 2017; 45: 1133-1138
        • Boyce JM.
        Alcohol as surface disinfectants in healthcare settings.
        Infect Control Hosp Epidemiol. 2018; 39: 323-328
        • Becker B
        • Henningsen L
        • Paulmann D
        • et al.
        Evaluation of the virucidal efficacy of disinfectant wipes with a test method simulating practical conditions.
        Antimicrob Resist Infect Control. 2019; 8: 121
        • Frobisher Jr., M
        • Sommermeyer L
        • Blackwell MJ
        Studies on disinfection of clinical thermometers. I. Oral thermometers.
        Appl Microbiol. 1953; 1: 187-194
        • Best M
        • Sattar SA
        • Springthorpe VS
        • Kennedy ME
        Efficacies of selected disinfectants against Mycobacterium tuberculosis.
        J Clin Microbiol. 1990; 28: 2234-2239
        • Larson E
        • Bobo L.
        Effective hand degerming in the presence of blood.
        J Emerg Med. 1992; 10: 7-11
        • Traore O
        • Springthorpe VS
        • Sattar SA
        Testing chemical germicides against Candida species using quantitative carrier and fingerpad methods.
        J Hosp Infect. 2002; 50: 66-75
        • Sattar SA.
        Microbicides and the environmental control of nosocomial viral infections.
        J Hosp Infect. 2004; 56: S64-S69
        • Rabenau HF
        • Kampf G
        • Cinatl J
        • Doerr HW
        Efficacy of various disinfectants against SARS coronavirus.
        J Hosp Infect. 2005; 61: 107-111
        • Magulski T
        • Paulmann D
        • Bischoff B
        • et al.
        Inactivation of murine norovirus by chemical biocides on stainless steel.
        BMC Infect Dis. 2009; 9: 107
        • Jeong EK
        • Bae JE
        • Kim IS
        Inactivation of influenza A virus H1N1 by disinfection process.
        Am J Infect Control. 2010; 38: 354-360
        • Rutala WA
        • Kanamori H
        • Gergen MF
        • Sickbert-Bennett EE
        • Weber DJ
        Susceptibility of Candida auris and Candida albicans to 21 germicides used in healthcare facilities.
        Infect Control Hosp Epidemiol. 2019; 40: 380-382
        • Foddai AC
        • Grant IR
        • Dean M
        Efficacy of instant hand sanitizers against foodborne pathogens compared with hand washing with soap and water in food preparation settings: asystematic review.
        J Food Prot. 2016; 79: 1040-1054
        • Gonzalez EA
        • Nandy P
        • Lucas AD
        • Hitchins VM
        Ability of cleaning-disinfecting wipes to remove bacteria from medical device surfaces.
        Am J Infect Control. 2015; 43: 1331-1335
        • Meinke R
        • Meyer B
        • Frei R
        • Passweg J
        • Widmer AF
        Equal efficacy of glucoprotamin and an aldehyde product for environmental disinfection in a hematologic transplant unit: a prospective crossover trial.
        Infect Control Hosp Epidemiol. 2012; 33: 1077-1080
        • Kampf G
        • Degenhardt S
        • Lackner S
        • Jesse K
        • von BH
        • Ostermeyer C
        Poorly processed reusable surface disinfection tissue dispensers may be a source of infection.
        BMC Infect Dis. 2014; 14: 37
        • Gant VA
        • Jeanes A
        • Hall TJ
        Response to: Griffith CJ, Dancer SJ. 'Hospital cleaning: problems with steam cleaning and microfibre.
        J Hosp Infect. 2010; 74: 82-84
        • Goldsmith MT
        • Latlief MA
        • Friedl JL
        • Stuart LS
        Adsorption of available chlorine and quaternary by cotton and wool fabrics from disinfecting solutions.
        Appl Microbiol. 1954; 2: 360-364
        • Bloss R
        • Meyer S
        • Kampf G
        Adsorption of active ingredients of surface disinfectants depends on the type of fabric used for surface treatment.
        J Hosp Infect. 2010; 75: 56-61
        • Engelbrecht K
        • Ambrose D
        • Sifuentes L
        • Gerba C
        • Weart I
        • Koenig D
        Decreased activity of commercially available disinfectants containing quaternary ammonium compounds when exposed to cotton towels.
        Am J Infect Control. 2013; 41: 908-911
        • Hinchliffe DJ
        • Condon BD
        • Slopek RP
        • Reynolds M
        The adsorption of alkyl-dimethl-benzyl-ammonium chloride onto cotton nonwoven hydroentangled substrates at the solid-liquid interface is minimized by additive chemistries.
        Textile Res J. 2017; 87: 70-80
        • Wesgate R
        • Robertson A
        • Barrell M
        • Teska P
        • Maillard JY
        Impact of test protocols and material binding on the efficacy of antimicrobial wipes.
        J Hosp Infect. 2019; 103: e25-e32
        • Hong Y
        • Teska PJ
        • Oliver HF
        Effects of contact time and concentration on bactericidal efficacy of 3 disinfectants on hard nonporous surfaces.
        Am J Infect Control. 2017; 45: 1284-1285
        • Song X
        • Vossebein L
        • Zille A
        Efficacy of disinfectant-impregnated wipes used for surface disinfection in hospitals: a review.
        Antimicrob Resist Infect Control. 2019; 8: 139
        • Trajtman AN
        • Manickam K
        • Alfa MJ
        Microfiber cloths reduce the transfer of Clostridium difficile spores to environmental surfaces compared with cotton cloths.
        Am J Infect Control. 2015; 43: 686-689
        • Cadnum JL
        • Hurless KN
        • Kundrapu S
        • Donskey CJ
        Transfer of Clostridium difficile spores by nonsporicidal wipes and improperly used hypochlorite wipes: practice + product = perfection.
        Infect Control Hosp Epidemiol. 2013; 34: 441-442
        • Tuladhar E
        • Hazeleger WC
        • Koopmans M
        • Zwietering MH
        • Beumer RR
        • Duizer E
        Residual viral and bacterial contamination of surfaces after cleaning and disinfection.
        Appl Environ Microbiol. 2012; 78: 7769-7775
        • West AM
        • Teska PJ
        • Oliver HF
        There is no additional bactericidal efficacy of environmental protection agency-registered disinfectant towelettes after surface drying or beyond label contact time.
        Am J Infect Control. 2019; 47: 27-32
        • Carter Y
        • Barry D.
        Tackling C. difficile with environmental cleaning.
        Nurs Times. 2011; 107: 22-25
        • Boyce JM
        • Havill NL.
        Evaluation of a new hydrogen peroxide wipe disinfectant.
        Infect Control Hosp Epidemiol. 2013; 34: 521-523
        • Boyce JM
        • Guercia KA
        • Sullivan L
        • et al.
        Prospective cluster controlled crossover trial to compare the impact of an improved hydrogen peroxide disinfectant and a quaternary ammonium-based disinfectant on surface contamination and health care outcomes.
        Am J Infect Control. 2017; 45: 1006-1010
        • Hu H
        • Johani K
        • Gosbell IB
        • et al.
        Intensive care unit environmental surfaces are contaminated by multidrug-resistant bacteria in biofilms: combined results of conventional culture, pyrosequencing, scanning electron microscopy, and confocal laser microscopy.
        J Hosp Infect. 2015; 91: 35-44
        • Ledwoch K
        • Dancer SJ
        • Otter JA
        • et al.
        Beware biofilm! Dry biofilms containing bacterial pathogens on multiple healthcare surfaces; a multi-centre study.
        J Hosp Infect. 2018; 100: e47-e56
        • Vickery K
        • Deva A
        • Jacombs A
        • Allan J
        • Valente P
        • Gosbell IB
        Presence of biofilm containing viable multiresistant organisms despite terminal cleaning on clinical surfaces in an intensive care unit.
        J Hosp Infect. 2012; 80: 52-55
        • Almatroudi A
        • Gosbell IB
        • Hu H
        • et al.
        Staphylococcus aureus dry-surface biofilms are not killed by sodium hypochlorite: implications for infection control.
        J Hosp Infect. 2016; 93: 263-270
        • Chowdhury D
        • Rahman A
        • Hu H
        • Jensen SO
        • Deva AK
        • Vickery K
        Effect of disinfectant formulation and organic soil on the efficacy of oxidizing disinfectants against biofilms.
        J Hosp Infect. 2019; 103: e33-e41
        • Ledwoch K
        • Said J
        • Norville P
        • Maillard JY
        Artificial dry surface biofilm models for testing the efficacy of cleaning and disinfection.
        Lett Appl Microbiol. 2019; 68: 329-336
        • Carling PC
        • Parry MM
        • Rupp ME
        • Po JL
        • Dick B
        • Von BS
        Improving cleaning of the environment surrounding patients in 36 acute care hospitals.
        Infect Control Hosp Epidemiol. 2008; 29: 1035-1041
        • Boyce JM
        • Havill NL
        • Havill HL
        • Mangione E
        • Dumigan DG
        • Moore BA
        Comparison of fluorescent marker systems with 2 quantitative methods of assessing terminal cleaning practices.
        Infect Control Hosp Epidemiol. 2011; 32: 1187-1193
        • Berendt AE
        • Turnbull L
        • Spady D
        • Rennie R
        • Forgie SE
        Three swipes and you're out: how many swipes are needed to decontaminate plastic with disposable wipes?.
        Am J Infect Control. 2011; 39: 442-443
        • Boyce JM
        • Havill NL
        • Lipka A
        • Havill H
        • Rizvani R
        Variations in hospital daily cleaning practices.
        Infect Control Hosp Epidemiol. 2010; 31: 99-101
        • Rupp ME
        • Huerta T
        • Cavalieri RJ
        • et al.
        Optimum outlier model for potential improvement of environmental cleaning and disinfection.
        Infect Control Hosp Epidemiol. 2014; 35: 721-723
        • Tyan K
        • Jin K
        • Kang J
        A novel color additive for bleach wipes indicates surface coverage and contact time to improve thoroughness of cleaning.
        Infect Control Hosp Epidemiol. 2019; 40: 256-258
        • Mustapha A
        • Cadnum JL
        • Alhmidi H
        • Donskey CJ
        Evaluation of novel chemical additive that colorizes chlorine-based disinfectants to improve visualization of surface coverage.
        Am J Infect Control. 2018; 46: 119-121
        • Rathod SN
        • Beauvais K
        • Sullivan LK
        • Sudikoff SN
        • Peaper DR
        • Martinello RA
        The effectiveness of a novel colorant additive in the daily cleaning of patient rooms.
        Infect Control Hosp Epidemiol. 2019; 40: 721-723
        • Kundrapu S
        • Sunkesula V
        • Jury LA
        • Sitzlar BM
        • Donskey CJ
        Daily disinfection of high-touch surfaces in isolation rooms to reduce contamination of healthcare workers' hands.
        Infect Control Hosp Epidemiol. 2012; 33: 1039-1042
        • Sitzlar B
        • Deshpande A
        • Fertelli D
        • Kundrapu S
        • Sethi AK
        • Donskey CJ
        An environmental disinfection odyssey: evaluation of sequential interventions to improve disinfection of Clostridium difficile isolation rooms.
        Infect Control HospEpidemiol. 2013; 34: 459-465
        • Deshpande A
        • Donskey CJ.
        Practical approaches for assessment of daily and post-discharge room disinfection in healthcare facilities.
        Curr Infect Dis Rep. 2017; 19: 32
        • Gebel J
        • Exner M
        • French G
        • et al.
        The role of surface disinfection in infection prevention.
        GMS Hyg Infect Control. 2013; 8: Doc10
        • Rock C
        • Cosgrove SE
        • Keller SC
        • et al.
        Using a human factors engineering approach to improve patient room cleaning and disinfection.
        Infect Control Hosp Epidemiol. 2016; 37: 1502-1506
        • Anderson DJ
        • Chen LF
        • Weber DJ
        • Moehring RW
        • Lewis SS
        • Triplett PF
        • et al.
        Enhanced terminal room disinfection and acquisition and infection caused by multidrug-resistant organisms and Clostridium difficile (the Benefits of Enhanced Terminal Room Disinfection study): a cluster-randomised, multicentre, crossover study.
        Lancet. 2017; 389: 805-814
        • Ali S
        • Moore G
        • Wilson AP
        Effect of surface coating and finish upon the cleanability of bed rails and the spread of Staphylococcus aureus.
        J Hosp Infect. 2012; 80: 192-198
        • Rutala WA
        • Weber DJ
        Surface disinfection: treatment time (wipes and sprays) versus contact time (Liquids).
        Infect Control HospEpidemiol. 2018; 39: 329-331
        • Teska PJ
        • Li X
        • Gauthier J
        Wet contact time directly impacts antimicrobial efficacy of environmental protection agency-registered disinfectants.
        Am J Infect Control. 2019; 47: 474-476
      4. Environmental Protection Agency. EPA MLB SOP-MB-31: Procedure for the OECD quantitative method for testing antmicrobial products against Clostridium difficile (ATCC 43598) on inanimate, hard, non-porous surfaces. 2017. Available at: https://www.epa.gov/sites/production/files/2017-12/documents/mb-31_december_2017.pdf. Accessed June 13, 2020.

        • Reiss I
        • Borkhardt A
        • Fussle R
        • Sziegoleit A
        • Gortner L
        Disinfectant contaminated with Klebsiella oxytoca as a source of sepsis in babies.
        Lancet. 2000; 356: 310
        • Kupfahl C
        • Walther M
        • Wendt C
        • von BH
        Identical achromobacter strain in reusable surface disinfection tissue dispensers and a clinical isolate.
        Infect Control Hosp Epidemiol. 2015; 36: 1362-1364
        • Boyce JM.
        Modern technologies for improving cleaning and disinfection of environmental surfaces in hospitals.
        Antimicrob Resist Infect Control. 2016; 5: 10
        • O'Neill C
        • Ramage L
        • Wyatt L
        • Ballantyne L
        Quality control is indispensable for automated dilution systems with accelerated hydrogen peroxide.
        Can J Infect Control. 2009; 24: 226-228
        • Vetter L
        • Schuepfer G
        • Kuster SP
        • Rossi M
        A Hospital-wide outbreak of Serratiamarcescens, and Ishikawa's "Fishbone" analysis to support outbreak control.
        Qual Manag Health Care. 2016; 25: 1-7
        • Holm SM
        • Leonard V
        • Durrani T
        • Miller MD
        Do we know how best to disinfect child care sites in the United States? A review of available disinfectant efficacy data and health risks of the major disinfectant classes.
        Am J Infect Control. 2019; 47: 82-91
        • Alfa MJ
        • Lo E
        • Wald A
        • Dueck C
        • Degagne P
        • Harding GK
        Improved eradication of Clostridium difficile spores from toilets of hospitalized patients using an accelerated hydrogen peroxide as the cleaning agent.
        BMC Infect Dis. 2010; 10: 268
        • Rutala WA
        • Gergen MF
        • Sickbert-Bennett EE
        • Williams DA
        • Weber DJ
        Effectiveness of improved hydrogen peroxide in decontaminating privacy curtains contaminated with multidrug-resistant pathogens.
        Am J Infect Control. 2014; 42: 426-428
        • Meakin NS
        • Bowman C
        • Lewis MR
        • Dancer SJ
        Comparison of cleaning efficacy between in-use disinfectant and electrolysed water in an English residential care home.
        J Hosp Infect. 2012; 80: 122-127
        • Attaway III, HH
        • Fairey S
        • Steed LL
        • Salgado CD
        • Michels HT
        • Schmidt MG
        Intrinsic bacterial burden associated with intensive care unit hospital beds: effects of disinfection on population recovery and mitigation of potential infection risk.
        Am J Infect Control. 2012; 40: 907-912
        • Casini B
        • Righi A
        • De FN
        • et al.
        Improving cleaning and disinfection of high-touch surfaces in intensive care during carbapenem-resistant Acinetobacter baumannii endemo-epidemic situations.
        Int J Environ Res Public Health. 2018; 15: 2305
        • Schmidt MG
        • Fairey SE
        • Attaway HH
        In situ evaluation of a persistent disinfectant provides continuous decontamination within the clinical environment.
        Am J Infect Control. 2019; 47: 732-734
        • Mayfield JL
        • Leet T
        • Miller J
        • Mundy LM
        Environmental control to reduce transmission of Clostridium difficile.
        Clin Infect Dis. 2000; 31: 995-1000
        • Hacek DM
        • Ogle AM
        • Fisher A
        • Robicsek A
        • Peterson LR
        Significant impact of terminal room cleaning with bleach on reducing nosocomial Clostridium difficile.
        Am J Infect Control. 2010; 38: 350-353
        • Orenstein R
        • Aronhalt KC
        • McManus Jr., JE
        • Fedraw LA
        A targeted strategy to wipe out Clostridium difficile.
        Infect Control Hosp Epidemiol. 2011; 32: 1137-1139
        • Alfa MJ
        • Lo E
        • Olson N
        • MacRae M
        • Buelow-Smith L
        Use of a daily disinfectant cleaner instead of a daily cleaner reduced hospital-acquired infection rates.
        Am J Infect Control. 2015; 43: 141-146
        • Dagher D
        • Ungar K
        • Robison R
        • Dagher F
        The wide spectrum high biocidal potency of Bioxy formulation when dissolved in water at different concentrations.
        PLoS One. 2017; 12e0172224
        • Gonzalez M
        • Jegu J
        • Kopferschmitt MC
        • et al.
        Asthma among workers in healthcare settings: role of disinfection with quaternary ammonium compounds.
        Clin Exp Allergy. 2014; 44: 393-406
        • Gonzalez EA
        • Nandy P
        • Lucas AD
        • Hitchins VM
        Designing for cleanability: the effects of material, surface roughness, and the presence of blood test soil and bacteria on devices.
        Am J Infect Control. 2017; 45: 194-196
        • Gold KM
        • Hitchins VM.
        Cleaning assessment of disinfectant cleaning wipes on an external surface of a medical device contaminated with artificial blood or Streptococcus pneumoniae.
        Am J Infect Control. 2013; 41: 901-907
        • Lopez GU
        • Kitajima M
        • Havas A
        • Gerba CP
        • Reynolds KA
        Evaluation of a disinfectant wipe intervention on fomite-to-finger microbial transfer.
        Appl Environ Microbiol. 2014; 80: 3113-3118
        • Wallace RL
        • Ouellette M
        • Jean J
        Effect of UV-C light or hydrogen peroxide wipes on the inactivation of methicillin-resistant Staphylococcus aureus, Clostridium difficile spores and norovirus surrogate.
        J Appl Microbiol. 2019; 127: 586-597