Highlight
- •ESKAPE bacteria with biofilm-forming capacity.
- •Ozone showed bactericidal activity on bacteria ESKAPE.
- •Ozone as a disinfectant alternative.
Abstract
Background
Medical devices can be reservoirs of multidrug-resistant bacteria that may be involved
in the acquisition of infections since bacteria with the ability to form biofilms
that are difficult to eradicate, mainly in mechanical ventilators. The aim of this
work was to evaluate the efficacy of O3 against biofilms of bacteria ESKAPE group through disinfection studies.
Methods
The formation of biofilms of ESKAPE group bacteria was induced in vitro. O3 was injected at different exposure times at a constant dose of 600 mg/h. The recovery
of surviving bacteria after O3 treatment was assessed by bacterial counts and biofilm disruption was analyzed. Finally,
the viability and integrity of biofilms after O3 treatment was determined by confocal laser scanning microscopy (CLSM).
Results
O3 showed bactericidal activity on biofilms from 12 min/7.68 ppm for A. baumannii and C. freundii. P. aeruginosa, K. pneumoniae and S. aureus were killed after 15 min/9.60 ppm. Correlation analyses showed inversely proportional
relationships between the variables "disruption versus O3". CLSM revealed that death was time-dependent of biofilms upon O3 exposure. Orthogonal plane analysis showed that bacteria located in the outer region
of the biofilms were the ones that initially suffered damage from O3 exposure.
Conclusions
Our findings suggest that this method could be an alternative for the disinfection
in mechanical ventilators colonized by bacteria biofilm forming.
Keywords
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Article info
Publication history
Published online: April 25, 2022
Footnotes
Funding/support: This study is part of the project “CONACyT 313771: Análisis del efecto del ozono sobre SARS-CoV-2 como alternativa de producto desinfectante en equipos de protección del personal de salud de alta demanda” and funding of the 022 budget (years 2020, 2021) of the “Hospital Juárez de México” and partially funded by the “CONACyT 300461 to Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez”.
Conflict of interest: None to report.
Identification
Copyright
© 2022 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.
