Highlights
- •Nanotechnology against multidrug-resistant Acinetobacter baumannii.
- •Development of a nanodrug for the treatment of multidrug-resistant strains.
- •Antimicrobial based on AgNPs and aminoglycosides against multidrug-resistant.
Abstract
Background
Multidrug-resistant bacteria are one of the world's biggest health problems; therefore,
improving the spectrum of action of antibiotics could be necessary to reverse this
situation. Amikacin and silver salts have well-known antimicrobial properties. However,
both drugs lost their effectiveness against some bacteria, such as Acinetobacter baumannii. This work aims to develop a nanodrug from silver nanoparticles (AgNPs) functionalized
with Amikacin against multidrug-resistant Acinetobacter baumannii.
Methods
AgNPs were produced using the bottom-up methodology and functionalized with Amikacin
modified by the carbodiimide-based chemistry, forming [email protected] Susceptibility
tests were performed using Amikacin-resistant Acinetobacter baumannii strains to assess the bacteriostatic and bactericidal potential of the developed
nanodrug. The clinical strains were induced to form a biofilm, and biomass quantification
and the metabolic activity were determined.
Results
The AgNPs have a hydrodynamic diameter of the particles with a bimodal distribution,
with a size of 37.84 nm. The FT-IR spectrum of [email protected] exhibits vibrational
modes corresponding to Amikacin, confirming the conjugation to AgNPs. Susceptibility
testing demonstrated a minimal inhibitory and bactericidal concentration of < 0.5
µg/mL. The [email protected] reduced the biofilm metabolic activity of Acinetobacter baumannii at rates ≥ 50%, characterized by the minimal biofilm inhibition concentrations.
Conclusions
Results demonstrate a promising development of a new nanodrug with lower concentrations,
less toxicity, and greater efficacy against multidrug-resistant Acinetobacter baumannii.
Key Words
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Article info
Publication history
Published online: December 25, 2022
Publication stage
In Press Journal Pre-ProofFootnotes
Conflict of interest: None to report.
Author contributions: L.O.C., conceptualization and design; acquisition, analysis, and interpretation of data for the work; and writing (original draft); I.F., acquisition, analysis, and interpretation of data for the work; T.S.V., acquisition, analysis, and interpretation of data for the work; L.R., conceptualization, acquisition, analysis, and interpretation of data for the work; M. L. C., conceptualization, design, analysis, interpretation of data for the work, validation, supervision, and writing (original draft).
Identification
Copyright
© 2023 Published by Elsevier Inc. on behalf of Association for Professionals in Infection Control and Epidemiology, Inc.
