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Antibacterial activity of silver nanoparticles functionalized with amikacin applied against multidrug-resistant acinetobacter baumannii

Published:December 25, 2022DOI:https://doi.org/10.1016/j.ajic.2022.12.009

      Highlights

      • Nanotechnology against multidrug-resistant Acinetobacter baumannii.
      • Development of a nanodrug for the treatment of multidrug-resistant strains.
      • Antimicrobial based on AgNPs and aminoglycosides against multidrug-resistant.

      Abstract

      Background

      Multidrug-resistant bacteria are one of the world's biggest health problems; therefore, improving the spectrum of action of antibiotics could be necessary to reverse this situation. Amikacin and silver salts have well-known antimicrobial properties. However, both drugs lost their effectiveness against some bacteria, such as Acinetobacter baumannii. This work aims to develop a nanodrug from silver nanoparticles (AgNPs) functionalized with Amikacin against multidrug-resistant Acinetobacter baumannii.

      Methods

      AgNPs were produced using the bottom-up methodology and functionalized with Amikacin modified by the carbodiimide-based chemistry, forming [email protected] Susceptibility tests were performed using Amikacin-resistant Acinetobacter baumannii strains to assess the bacteriostatic and bactericidal potential of the developed nanodrug. The clinical strains were induced to form a biofilm, and biomass quantification and the metabolic activity were determined.

      Results

      The AgNPs have a hydrodynamic diameter of the particles with a bimodal distribution, with a size of 37.84 nm. The FT-IR spectrum of [email protected] exhibits vibrational modes corresponding to Amikacin, confirming the conjugation to AgNPs. Susceptibility testing demonstrated a minimal inhibitory and bactericidal concentration of < 0.5 µg/mL. The [email protected] reduced the biofilm metabolic activity of Acinetobacter baumannii at rates ≥ 50%, characterized by the minimal biofilm inhibition concentrations.

      Conclusions

      Results demonstrate a promising development of a new nanodrug with lower concentrations, less toxicity, and greater efficacy against multidrug-resistant Acinetobacter baumannii.

      Key Words

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      References

        • O’Neill J.
        Review on antimicrobial resistance: tackling drug-resistant infections globally: final report and recommendations.
        Rev Antimicrob Resist tackling drug-resistant Infect Glob Final Rep Recomm. 2016; 1: 80
        • Falavigna M
        • Colpani V
        • Stein C
        • et al.
        Guidelines for the pharmacological treatment of COVID-19. The task-force/consensus guideline of the Brazilian association of intensive care medicine, the Brazilian society of infectious diseases and the Brazilian society of pulmonology and tisiology.
        Rev Bras Ter intensiva. 2020; 32: 166-196
        • Tiri B
        • Sensi E
        • Marsiliani V
        • et al.
        Antimicrobial stewardship program, COVID-19, and infection control: spread of carbapenem-resistant klebsiella pneumoniae colonization in ICU COVID-19 patients. What did not work?.
        J Clin Med. 2020; 9: 2744
      1. National Health Surveillance Agency. Guidelines for the prevention and control of the spread of multidrug-resistant microorganisms in health services in the context of the COVID-19 pandemic [Internet]. Technical note 05/2021; 2021. Available from: https://www.gov.br/anvisa/pt-br/centraisdeconteudo/publicacoes/servicosdesaude/notas-tecnicas/nota-tecnica-gvims-ggtes-anvisa-no-05-2021

        • Eckardt P
        • Canavan K
        • Guran R
        • et al.
        Containment of a carbapenem-resistant Acinetobacter baumannii complex outbreak in a COVID-19 intensive care unit.
        Am J Infect Control. 2022; 50: 477-481
        • Brunton LL
        • Lazo JS
        • Parker KL.
        The Pharmacological Basis of Therapeutics.
        Goodman, Gilmans, Ed, 2006: 11
        • Munita JM
        • Arias CA.
        Mechanisms of antibiotic resistance.
        Microbiol Spectr. 2016; 4: 2-4
        • Marra AR
        • Camargo LFA
        • Pignatari ACC
        • et al.
        Nosocomial bloodstream infections in Brazilian hospitals: analysis of 2,563 cases from a prospective nationwide surveillance study.
        J Clin Microbiol. 2011; 49: 1866-1871
        • Franci G
        • Falanga A
        • Galdiero S
        • et al.
        Silver nanoparticles as potential antibacterial agents.
        Molecules. 2015; 20: 8856-8874
        • Mulfinger L
        • Solomon SD
        • Bahadory M
        • Jeyarajasingam A V
        • Rutkowsky SA
        • Boritz C.
        Synthesis and study of silver nanoparticles.
        J Chem Educ. 2007; 84: 322
        • Agnihotri S
        • Mukherji S
        • Mukherji S.
        Size-controlled silver nanoparticles synthesized over the range 5–100 nm using the same protocol and their antibacterial efficacy.
        Rsc Adv. 2014; 4: 3974-3983
        • Jesus VPS
        • Raniero L
        • Lemes GM
        • Bhattacharjee TT
        • Júnior PCC
        • Castilho ML.
        Nanoparticles of methylene blue enhance photodynamic therapy.
        Photodiagnosis Photodyn Ther. 2018; 23: 212-217
        • Salunke GR
        • Ghosh S
        • Kumar RJS
        • et al.
        Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control.
        Int J Nanomedicine. 2014; 9: 2635
        • Hanaor D
        • Michelazzi M
        • Leonelli C
        • Sorrell CC.
        The effects of carboxylic acids on the aqueous dispersion and electrophoretic deposition of ZrO2.
        J Eur Ceram Soc. 2012; 32: 235-244
        • Lopez-Carrizales M
        • Velasco KI
        • Castillo C
        • et al.
        In vitro synergism of silver nanoparticles with antibiotics as an alternative treatment in multiresistant uropathogens.
        Antibiotics. 2018; 7: 50
        • Castilho ML
        • Hewitt KC
        • Raniero L.
        FT-IR characterization of a theranostic nanoprobe for photodynamic therapy and epidermal growth factor receptor targets.
        Sens Actua B Chem. 2017 Mar; 240 ([Internet]. Available from:): 903-908
        • Coates J
        Interpretation of Infrared Spectra, a Practical Approach. R.A. Meyer. Encyclopedia ofAnalytical Chemistry.
        John Wiley & Sons, 2006: 10815-10837
        • Fatima S
        • Panda AK
        • Talegaonkar S
        • Iqbal Z
        • Ahmad FJ.
        Optimization and designing of amikacin-loaded poly d, l-lactide-co-glycolide nanoparticles for effective and sustained drug delivery.
        J Pharm Bioallied Sci. 2019; 11: 83
        • Kumar S
        • Meena VK
        • Hazari PP
        • Sharma SK
        • Sharma RK.
        Rose Bengal attached and dextran coated gadolinium oxide nanoparticles for potential diagnostic imaging applications.
        Eur J Pharm Sci. 2018; 117 ([Internet]. Available from:): 362-370
        • Garcia-Fuentes M
        • Giger E
        • Meinel L
        • Merkle HP.
        The effect of hyaluronic acid on silk fibroin conformation.
        Biomaterials. 2008; 29: 633-642
        • Rozenberg M
        • Lansky S
        • Shoham Y
        • Shoham G.
        Spectroscopic FTIR and NMR study of the interactions of sugars with proteins.
        Spectrochim Acta Part A Mol Biomol Spectrosc. 2019 Nov; 222 ([Internet]. Available from:)116861
        • El-Bassyouni GT
        • Eldera SS
        • Kenawy SH
        • Hamzawy EMA.
        Hydroxyapatite nanoparticles derived from mussel shells for in vitro cytotoxicity test and cell viability.
        Heliyon. 2020; 6: e04085
        • Bury-Moné S.
        Antibacterial Therapeutic Agents.
        Reference Module in Biomedical Sciences. Elsevier, 2014 ([Internet]. Available from:)
        • Kaur A
        • Kumar R.
        Enhanced bactericidal efficacy of polymer stabilized silver nanoparticles in conjugation with different classes of antibiotics.
        RSC Adv. 2019; 9: 1095-1105
        • Łysakowska ME
        • Ciebiada-Adamiec A
        • Klimek L
        • Sienkiewicz M.
        The activity of silver nanoparticles (axonnite) on clinical and environmental strains of acinetobacter spp.
        Burns. 2015; 41 (Available from): 364-371https://doi.org/10.1016/j.burns.2014.07.014
        • Singh R
        • Vora J
        • Nadhe SB
        • Wadhwani SA
        • Shedbalkar UU
        • Chopade BA.
        Antibacterial activities of bacteriagenic silver nanoparticles against nosocomial Acinetobacter baumannii.
        J Nanosci Nanotechnol. 2018; 18: 3806-3815
        • Li Z
        • Ding Z
        • Liu Y
        • et al.
        Phenotypic and genotypic characteristics of biofilm formation in clinical isolates of Acinetobacter baumannii.
        Infect Drug Resist. 2021; 14: 2613-2624
        • Latka A
        • Drulis-Kawa Z.
        Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase.
        Sci Rep. 2020; 10: 1-12
        • Rabin N
        • Zheng Y
        • Opoku-Temeng C
        • Du Y
        • Bonsu E
        • Sintim HO.
        Agents that inhibit bacterial biofilm formation.
        Future Med Chem. 2015; 7: 647-671
        • Amin M
        • Navidifar T
        • Shooshtari FS
        • et al.
        Association between biofilm formation, structure, and the expression levels of genes related to biofilm formation and biofilm-specific resistance of Acinetobacter baumannii strains isolated from burn infection in Ahvaz.
        Iran. Infect Drug Resist. 2019; 12: 3867
        • Lin MF
        • Lin YY
        • Lan CY.
        Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation.
        PeerJ. 2020; 2020: 1-20
        • Hetta HF
        • Al-Kadmy IMS
        • Khazaal SS
        • et al.
        Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii.
        Sci Rep. 2021; 11 ([Internet]Available from): 1-11https://doi.org/10.1038/s41598-021-90208-4